Identification of metallothionein protein in Anodonta woodiana as a biomarker of mercury (Hg) contamination

Abstract. Rahayu SYS, Fadila A, Fahmi MR. 2023. Identification of metallothionein protein in Anodonta woodiana as a biomarker mercury (Hg) contamination. Nusantara Bioscience 15: 90-94. Heavy metal contamination can affect the survival aquatic biota and will accumulate bodiesorganisms. Moreover, identification at molecular level be analyzed using analysis. Biomarkers are responses measured individually, ranging from enzymes xenobiotic measurements to organ overall conditions. Biomarker analysis done by checking protein, this expression induced Reactive Oxygen Species (ROS). Metallothionein (MT) has thiol group with nucleophilic properties. As result, make able find metals free radicals. Therefore, prevention that reduce higher trophic requires monitoring observing protein. For example, (Rea, 1834) or kijing taiwan HgCl2 aims characterize their absorption ability environment through That was conducted SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) method. electrophoresis results showed size 7 kDa, 15 >30 kDa. Size kDa is proteins related stress control Heat shock (Hsp). The presence Hsp due body increasing synthesis normal synthesis. Based on obtained, study revealed A. could absorb HgCl2, evidenced characterization results.